Base de Caldo de Enriquecimento para Listeria 500g - Biocomma

Código: GF1005F Marca: Biocomma

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DESCRIPTION: Listeria Enrichment Broth Base
APPLICATlON: For the selective enrichment of Listeria.

PRODUCT NAME	Listeria Enrichment Broth Base
PRODUCT CODE	GF1005F
DESCRIPTION	Listeria Enrichment Broth Base
APPLICATION	For the selective enrichment of Listeria.
PREPARATION	1. Suspend 49.4 g in 1 L of purified water.
	2. Heat with frequent agitation and boil to completely dissolve the powder. Dispense into conical flasks, and autoclave it at 121 ℃ for 15min.
	3. Cool the sterilized medium to about 45°C, add 1 tube of LB1 reagent (2.7 mg of acriflavin hydrochloride and 4.5 mg of nalidixic acid) per 225 mL of base, and add 1 tube of LB2 reagent (5.0 mg of acriflavin hydrochloride and 4.0 mg of nalidixic acid) per 200 mL of base.
COMPOSITION	Composition	Amount（g/L）
	Tryptone	5
	Polyptone	5
	Yeast Extract	5
	Sodium Chloride	20
	Potassium Dihydrogen Phosphate	1.35
	Disodium Hydrogen Phosphate	12
	Esculin	1
	Final pH (25℃)	7.2±0.2
STORAGE	Store in the dark at room temperature before use.
SHELF LIFE	Shelf life of sealed product is 3 years.
PRECAUTIONS	1. NOT for clinical use.
	2. Do not use this product if it is lumpy.
	3. After use, all contaminated materials must be autoclaved at 121°C for 30 minutes and then disposed of.
	4.Pay attention to dust when weighing and wear a mask to avoid causing discomfort to the respiratory system.